β-Bisabolene   中文名称: β-红没药烯

Natural Sesquiterpene

β-红没药烯对小鼠细胞具有特异性细胞毒作用，对正常 Eph4、MG1361 和 4T1 细胞的 IC50 值分别 >200 µg/ml、65.49 µg/ml 和 48.99 µg/ml。相对于正常 MCF-10A、MCF-7、MDA-MB-231、SKBR3 和 BT474，其 IC50 值为 114.3 µg/mL、66.91 µg/mL、98.39 µg/mL、70.62 µg/mL 和 74.3 µg/mL细胞，分别[1]。

据观察，β-红没药烯可有效抑制体内移植的 4T1 乳腺肿瘤的生长（终点时体积减少 37.5%）[1]。

气相色谱-质谱（GC-MS）分析[1]
使用配备有MD 800质量选择性检测器和AS800 Finnegan自动进样器的Finnegan GC 8000气相色谱仪分析Opoponax提取物。对于毛细管柱，使用DB-5熔融二氧化硅柱，其尺寸如下：30×0.32 mm id和0.25µm膜厚。烘箱的温度以3°C/分钟的速度从50°C编程为240°C，并在该最终温度下保持2分钟。氦气载气的流速设定为1 ml/分钟，并保持在恒定压力下，同时注射器和气源的温度均设定为260°C。质量检测器用于正电子碰撞电离模式（EI+），电离电压为70eV。扫描范围为35-450质量单位，扫描时间为0.45s，用于获取扫描间时间为0.08s的质谱。

细胞活力测定[1]
对于活力测定，将鼠细胞以10000细胞/孔的密度铺板，将人细胞以20000细胞/孔铺板到96孔板中。细胞在接种后24小时用培养基中制备的相应试剂处理。然后在处理后24小时，根据制造商的说明，使用Cell Titer Blue对每个孔中的细胞活力进行定量。

---

细胞凋亡测定[1]
根据制造商的说明，用Caspase-Glo 3/7测定试剂盒分析指定处理后的细胞裂解物。根据制造商的说明，使用死细胞凋亡试剂盒进行膜联蛋白-V/碘化丙啶（PI）分析，并使用BD FACSCanto流式细胞仪分析染色细胞。

In vivo administration of β-bisabolene[1]
Of the oily compound β-bisabolene, solubilized in corn oil, 1.12 g/kg was administered intraperitoneally, two times a week, for 2 weeks, did not result in any signs of distress in all animals. Upon necropsy, the histology of liver, kidney, spleen and lung of these mice appeared normal (data not shown). When a dose of 2.24 g/kg was administered, mice showed signs of morbidity after single treatment. Hence, we used a maximum non-lethal dose of 1.12 g/kg β-bisabolene in our tumour growth studies. The treatment regime consisted of intraperitoneal (i.p.) injections twice weekly, once palpable tumour was detected (at 2 weeks) and until mice have reached the endpoint of the experiment or showed signs of morbidity.

---

Orthotopic cell transplants and tumour measurements[1]
All animal experiments were conducted in accordance with the UK Animals Act 1986, under Home Office licence 30/2849. 4T1 cells were trypsinized and dissociated into single-cell suspensions before transplantation into the abdominal mammary fat pads of wild-type BALB/C mice that were between 8 and 12 weeks old. Mice were then checked for palpable tumours, and the resulting tumours were measured using calipers three times weekly. At appropriate experimental endpoints, tumour, mammary gland, lung and liver tissues were harvested. Tissue samples were then fixed in 4% formaldehyde at 4 °C overnight before processing for histological analysis.

---

Immunohistology[1]
Fixed tissues were embedded in paraffin, sectioned into 5-µm slices, mounted onto poly-L-lysine-coated slides and stained with haematoxylin and eosin. Antibodies for cleaved caspase-3 and Ki-67 (Vector Labs) were used for immunohistology according to the manufacturer's instructions.

[1]. β-Bisabolene, a Sesquiterpene From the Essential Oil Extract of Opoponax (Commiphora Guidottii), Exhibits Cytotoxicity in Breast Cancer Cell Lines. Phytother Res. 2016 Mar;30(3):418-25.

(S)-beta-bisabolene is a beta-bisabolene which has (1S)-configuration. It is an enantiomer of a (R)-beta-bisabolene.
beta-Bisabolene has been reported in Magnolia officinalis, Cymbopogon martinii, and other organisms with data available.

C15H24

204.35

204.188

495-61-4

10104370

Colorless to light yellow liquid

0.854g/cm3

275.441ºC at 760 mmHg

109.79ºC

0.009mmHg at 25°C

1.484

5.035

204.188

0

0

4

15

274

1

[#6]\[#6](-[#6])=[#6]\[#6]-[#6]-[#6](=[#6])-[#6]-1-[#6]-[#6]-[#6](-[#6])=[#6]-[#6]-1 |c:12|

XZRVRYFILCSYSP-OAHLLOKOSA-N

InChI=1S/C15H24/c1-12(2)6-5-7-14(4)15-10-8-13(3)9-11-15/h6,8,15H,4-5,7,9-11H2,1-3H3/t15-/m1/s1

(4S)-1-methyl-4-(6-methylhepta-1,5-dien-2-yl)cyclohexene

β-Bisabolene

2934.99.9001

Powder      -20°C    3 years

                     4°C     2 years

In solvent   -80°C    6 months

                  -20°C    1 month

注意： 请将本产品存放在密封且受保护的环境中，避免吸湿/受潮。

Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

DMSO: 100 mg/mL (489.36 mM)

配方 1 中的溶解度: ≥ 2.5 mg/mL (12.23 mM) (饱和度未知) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (这些助溶剂从左到右依次添加，逐一添加), 澄清溶液。
例如，若需制备1 mL的工作液，可将100 μL 25.0 mg/mL澄清DMSO储备液加入到400 μL PEG300中，混匀；然后向上述溶液中加入50 μL Tween-80，混匀；加入450 μL生理盐水定容至1 mL。
*生理盐水的制备：将 0.9 g 氯化钠溶解在 100 mL ddH₂O中，得到澄清溶液。

---

配方 2 中的溶解度: ≥ 2.5 mg/mL (12.23 mM) (饱和度未知) in 10% DMSO + 90% Corn Oil (这些助溶剂从左到右依次添加，逐一添加), 澄清溶液。
例如，若需制备1 mL的工作液，可将 100 μL 25.0 mg/mL 澄清 DMSO 储备液加入到 900 μL 玉米油中并混合均匀。

---

请根据您的实验动物和给药方式选择适当的溶解配方/方案：
1、请先配制澄清的储备液(如：用DMSO配置50 或 100 mg/mL母液(储备液));
2、取适量母液，按从左到右的顺序依次添加助溶剂，澄清后再加入下一助溶剂。以 下列配方为例说明 (注意此配方只用于说明，并不一定代表此产品 的实际溶解配方):
10% DMSO → 40% PEG300 → 5% Tween-80 → 45% ddH2O (或 saline);
假设最终工作液的体积为 1 mL, 浓度为5 mg/mL: 取 100 μL 50 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀/澄清；向上述体系中加入50 μL Tween-80，混合均匀/澄清；然后继续加入450 μL ddH2O (或 saline)定容至 1 mL；
3、溶剂前显示的百分比是指该溶剂在最终溶液/工作液中的体积所占比例;
4、 如产品在配制过程中出现沉淀/析出，可通过加热(≤50℃)或超声的方式助溶;
5、为保证最佳实验结果，工作液请现配现用！
6、如不确定怎么将母液配置成体内动物实验的工作液，请查看说明书或联系我们;
7、 以上所有助溶剂都可在 Invivochem.cn网站购买。