Cellulose carboxymethyl ether sodium   中文名称: 羧甲基纤维素钠

0.5%羧甲基纤维素钠溶液的制备
称量0.5g CMC Na，并将其溶解在100 ml ddH2O或盐水中（0.9 g NaCl溶于100 ml ddH2O中），获得澄清溶液。
注意
1.确保CMC Na溶液中没有固液分离，溶液清澈无颗粒。
2.CMC Na的完全溶解可能需要4小时或更长时间。
3.根据文献报道，CMC Na（口服；5%水溶液；1年）在大鼠中耐受性良好。[2]
4.雌性小鼠CMC Na的LD50为14 g/kg小鼠体重，相当于9.8 g/kg大鼠体重，因此根据Loomis标准（LD50为5-15g/kg大鼠体重）被归类为实际无毒。[3]

We aimed to evaluate the effects of the barrier agent sodium carboxymethyl cellulose (SCMC) with and without dexamethasone for the prevention of postoperative adhesion formation in a rat model of postoperative peritoneal adhesion. A total of 160 three-month old male and female Wistar rats underwent a laparotomy, and adhesions were induced by ileocecal abrasion. Rats were randomly assigned to 4 groups (n=40 each): group A, untreated; group B, treated with SCMC only; group C1, treated with SCMC + 3 mg dexamethasone, and group C2, treated with SCMC + 8 mg dexamethasone. After 12 days, adhesion formation and histopathological changes were compared. In groups A, B, C1, and C2, the mortality rates were 10, 5, 5, and 5%, respectively. In groups C1 and C2, the adhesions were filmy and easy to dissect and were milder compared with those in groups A and B. The total adhesion score in group C1 (3.38±0.49) was significantly lower than that of group B (6.01±0.57; P<0.01) or group A (8.01±0.67; P<0.05). There was no significant difference in adhesion formation between groups C1 and C2. Compared with groups A and B, groups C1 and C2 exhibited milder histopathological changes. SCMC in combination with dexamethasone can prevent adhesion formation and is a better barrier agent than SCMC alone. The safety and feasibility of SCMC in combination with dexamethasone to prevent adhesion formation after abdominal surgery warrants further clinical study.[1]

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Mice were divided into ten groups of negative control (aquadest), positive control (standard Na-CMC), each four dose variances of uncrosslinked and crosslinked Na-CMC (1,75; 3,5; 7; and 14 g/kg body weight). Three female mice were used for each group. All mice were fasted approximately 18 hours prior to dosing, and water was provided continuously. Pharmacological screening was conducted prior to the oral administration. After the administration, the mice mortality was observed for 14 days. Then, the LD50 value was determined by probit analysis. Besides, all mice were observed for the sign of toxicity at 0.5; 1; 2; 4; and 24 hours after administration (effects on central nervous system: motor activity, forelimb grip strength, grasping, tremor, convulsion, catalepsy, sedative, straub tail, tail pinch, toe pinch, pineal response, respiration, and autonomic nervous system: piloerection, salivation, lacrimation, abnormal urination, diarrhea). The body weight was also observed daily for 14 days. Resulted data was analysed statistically[2].

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Experiment rats of both male and female Wistar rats were divided into 4 groups, each group consisted of 6 rats. Group-I was the normal group. Group-II negative control group received aquadest. Group-III positive control group received standard Na-CMC suspension (1.25 g/kg body weight). Group IV experimental group received crosslinked Na-CMC (1.25g/kg body weight). The animals were dosed daily for 28 days. Finishing the 28 days period, haematological test (erythrocyte, leukocyte, hemoglobin, hematocrit), blood biochemical analysis (SGOT, SGPT, creatinine, BUN), organ index determination (liver, kidney, lung, brain, heart, and stomach), histopathological examination of liver, kidney, and lung were done for each group. The organs were taken and fixed in Bouin for 48 hours. Specimens were prepared by dehydration, washing, infiltration, embedding, slicing with microtome, and staining with hematoxylin and eosin. Then, the specimens were placed on the glass slides and examined under a microscope with 100 times magnification[2].

[1]. Dexamethasone and sodium carboxymethyl cellulose prevent postoperative intraperitoneal adhesions in rats. Braz J Med Biol Res. 2015 Apr;48(4):344-8.

[2]. Common Vehicles for Nonclinical Evaluation of Therapeutic Agents.

[3]. Toxicity Evaluation of Na-CMC synthesized from Cellulose of Water Hyacinth (Eichhornia crassipes (Mart.) Solms).ISSN: 0975-8585.

A cellulose derivative which is a beta-(1,4)-D-glucopyranose polymer. It is used as a bulk laxative and as an emulsifier and thickener in cosmetics and pharmaceuticals and as a stabilizer for reagents.
See also: Carboxymethylcellulose Sodium (annotation moved to); Croscarmellose Sodium (annotation moved to) ... View More ...

[C6H7O2(OH)2OCH2COONA]N

263.1976

240.084

C, 36.51; H, 6.13; Na, 8.73; O, 48.63

9004-32-4

Sodium carboxymethyl cellulose (Viscosity:800-1200 mPa.s);9004-32-4;Sodium carboxymethyl cellulose (Viscosity:5000-15000 mPa.s);9004-32-4;Sodium carboxymethyl cellulose (MW 250000);9004-32-4

6328154

White to off-white solid

1,6 g/cm3

274 °C (dec.)

158.350

158.35

6

8

5

17

169

0

[Na].O([H])C([H])(C([H])(C([H])([H])O[H])O[H])C([H])(C([H])(C([H])=O)O[H])O[H].O([H])C(C([H])([H])[H])=O |^1:0|

DPXJVFZANSGRMM-UHFFFAOYSA-N

InChI=1S/C6H12O6.C2H4O2.Na/c7-1-3(9)5(11)6(12)4(10)2-8;1-2(3)4;/h1,3-6,8-12H,2H2;1H3,(H,3,4)

cetic acid compound with 2,3,4,5,6-pentahydroxyhexanal (1:1), sodium

Cellulose carboxymethyl ether sodium; Sodium CMC; CMC Na; Carboxymethylcellulose sodium, Sodium carboxymethyl cellulose, Carboxymethyl cellulose sodium; Cellulose gum sodium, CMC salt, CMC sodium; SODIUM CARBOXYMETHYL CELLULOSE; sodium;2,3,4,5,6-pentahydroxyhexanal;acetate; Carboxymethylcellulose sodium (USP); Carboxymethylcellulose cellulose carboxymethyl ether; Celluvisc (TN); CHEMBL242021; SCHEMBL25311455;

2934.99.9001

Powder      -20°C    3 years

                     4°C     2 years

In solvent   -80°C    6 months

                  -20°C    1 month

Note: Please store this product in a sealed and protected environment, avoid exposure to moisture.

Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

DMSO : ~12.5 mg/mL
H2O : ~8.33 mg/mL

配方 1 中的溶解度: ≥ 1.25 mg/mL (Infinity mM) (饱和度未知) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (这些助溶剂从左到右依次添加，逐一添加), 澄清溶液。
例如，若需制备1 mL的工作液，可将100 μL 12.5 mg/mL澄清的DMSO储备液加入到400 μL PEG300中，混匀；再向上述溶液中加入50 μL Tween-80，混匀；然后加入450 μL生理盐水定容至1 mL。
*生理盐水的制备：将 0.9 g 氯化钠溶解在 100 mL ddH₂O中，得到澄清溶液。

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配方 2 中的溶解度: ≥ 1.25 mg/mL (Infinity mM) (饱和度未知) in 10% DMSO + 90% Corn Oil (这些助溶剂从左到右依次添加，逐一添加), 澄清溶液。
例如，若需制备1 mL的工作液，可将 100 μL 12.5 mg/mL 澄清 DMSO 储备液加入到 900 μL 玉米油中并混合均匀。

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请根据您的实验动物和给药方式选择适当的溶解配方/方案：
1、请先配制澄清的储备液(如：用DMSO配置50 或 100 mg/mL母液(储备液));
2、取适量母液，按从左到右的顺序依次添加助溶剂，澄清后再加入下一助溶剂。以 下列配方为例说明 (注意此配方只用于说明，并不一定代表此产品 的实际溶解配方):
10% DMSO → 40% PEG300 → 5% Tween-80 → 45% ddH2O (或 saline);
假设最终工作液的体积为 1 mL, 浓度为5 mg/mL: 取 100 μL 50 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀/澄清；向上述体系中加入50 μL Tween-80，混合均匀/澄清；然后继续加入450 μL ddH2O (或 saline)定容至 1 mL；
3、溶剂前显示的百分比是指该溶剂在最终溶液/工作液中的体积所占比例;
4、 如产品在配制过程中出现沉淀/析出，可通过加热(≤50℃)或超声的方式助溶;
5、为保证最佳实验结果，工作液请现配现用！
6、如不确定怎么将母液配置成体内动物实验的工作液，请查看说明书或联系我们;
7、 以上所有助溶剂都可在 Invivochem.cn网站购买。